We next injected har-1−/− strains with WT and FTD/ALS-linked CHCHD10 mutations (R15L and S59L) and chose strains that contained comparable BFP fluorescence and CHCHD10/BFP expression by immunoblotting (Fig. 2a,b). This evidence concerns the gene CHCHD10 and amyotrophic lateral sclerosis.