EGFR and neoplasm: To understand if we are able to generate Half DVD-Ig molecules to study the effect of anti-CD3 binding valency on redirected T cell cytotoxicity for tumor killing in vitro, we generated a Half anti-EGFR (outer domain)/anti-CD3 (inner domain) DVD-Ig molecule connected using a long-long (L-L) linker by including four mutations at CH3 domain and two cysteine mutations at the hinge region (Fig. 2A).