SPOP mutations have been found in TCGA prostate cancer (10.2%), but with ∼70% has altered either Tryptophan residue at 131 position or Phenylalanine residue at 133 position (Supplementary Fig. 4), while mutations of ZNF148 and EZH1 were commonly observed in melanoma as well with no hot mutation spot. This evidence concerns the gene EZH1 and Familial prostate cancer.