Mirt-1cN also achieved ∼60% silencing of ataxin 7 in the SH-SY5Y human neuroblastoma cell line 48 h post-transfection (P < 0.0005), comparable with an ataxin 7-targeting shRNA (shR-1) (Supplementary Figure S1E). Here, ATXN7 is linked to neuroblastoma.