Three different patterns of CD3+ and CD8+ cell infiltration could be discriminated: (1) high CD3+ and CD8+ cell density in tumor core (CT) and invasive margin (IM), (2) high CD3+ and CD8+ cell density in IM and low density in CT and (3) low CD3+ and CD8+ cell density in CT and IM as shown by CD3 immunohistochemistry in three different exemplary HNSCC (Figure 2A). The gene discussed is CD8A; the disease is head and neck squamous cell carcinoma.