IHC and immunofluorescence (IF) analysis of tumor sections with Rip4 knockdown showed increased phospho-serine 727 Stat3 (pSerStat3) and nuclear Stat3 levels (Figures 2b and c,Supplementary Figure S2a) but no difference in phospho-tyrosine 705 Stat3 (pTyrStat3) (Supplementary Figure S2a). The gene discussed is STAT3; the disease is neoplasm.