In the Type 2 diabetic rat model induced by HFD and low-dose of STZ, biotin supplementation exerted antioxidant, anti-hyperlipidaemic, anti-inflammatory and anti-hyperglycaemic effects, increasing the level of insulin, probably through modulation of PPAR-γ, IRS-1 and NF-κB proteins [99]. The gene discussed is IRS1; the disease is type 2 diabetes mellitus.