Missense mutations (R108K, T263P, A289V and G598V; R84K, T239P, A265V, and G574V in mature EGFR) in the extracellular domain of EGFR were also found in GBM and glioblastoma cell lines, which conferred anchorage-independent growth and tumorigenicity upon NIH 3T3 cells [113,121,122,123]. The gene discussed is EGFR; the disease is glioblastoma.