The aim of the study was to analyze the prevalence of unrecognized cases with positive diabetes-associated autoantibodies among patients initially diagnosed with T2D, to describe their metabolic features and characterize the alterations in high sensitivity C-reactive protein (hs-CRP), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNFα) in comparison with participants having negative autoantibodies and patients already known to meet the criteria for LADA. This evidence concerns the gene TNF and diabetes mellitus.