Of these, 149 genes were hypermethylated and downregulated and 62 genes were hypomethylated and upregulated including AXIN2, ID4, EBF1, SOX4, SOX7, TAL1, PMEPA1, SPRY1, CDK6, and MEIS1. Pathway analysis using the genes having negative correlation for DNA methylation and gene expression in CLL Vs. Here, SPRY1 is linked to B-cell chronic lymphocytic leukemia.