The status of hypomethylation of PAX9 among unmutated CLL was confirmed through bisulfite genome sequencing of CpG island 3 in close proximity to CpG110 (Fig. 1).While CpG island 7 did not reveal any significant difference in methylation levels, the average % methylation at CpG island 3 was found to be 52.74% in mutated while 24.72% among unmutated CLL. This evidence concerns the gene PAX9 and B-cell chronic lymphocytic leukemia.