To understand the mechanism of ENb-TRAIL function, we compared the efficacy of ENb-TRAIL with EGFR-targeted therapeutics (monoclonal antibody Cetuximab, ENb or Erlotinib) or with TRAIL alone at different doses in HT29 (colon), Calu1 (lung), and LN229 (glioma) tumor lines. The gene discussed is TNFSF10; the disease is glioma.