To understand the mechanism of ENb-TRAIL function, we compared the efficacy of ENb-TRAIL with EGFR-targeted therapeutics (monoclonal antibody Cetuximab, ENb or Erlotinib) or with TRAIL alone at different doses in HT29 (colon), Calu1 (lung), and LN229 (glioma) tumor lines. This evidence concerns the gene EGFR and central nervous system cancer.