RNA sequencing was used to identify DEGs in resistant wild A. kiusianus and susceptible cultivated A. officinalis 24 h after infection with P. asparagi. Functional annotation and KEGG pathway analysis showed that the group of up-regulated genes in A. kiusianus was enriched for metabolic pathways, biosynthesis of secondary metabolites, plant-pathogen interaction, transcriptional regulation, protein kinase signaling, phenylpropanoid and hormone biosynthesis and signaling. This evidence concerns the gene WEE1 and infection.