We found that phosphorylation of the NF-κB p65 subunit at serine residue 536 peaked 48 h after infection and began to decrease from 72 h in both Toledo and Towne virus-infected cells, while the phosphorylated p65 levels were higher during Toledo virus infection than during Towne virus infection (Fig 8A). The gene discussed is NFKB1; the disease is infection.