This drug inactivates CRL/SCF E3 ligase and results in the accumulation of a large amount of its substrates, leading to the suppression of tumor growth.20 Triggering cell cycle disturbance, apoptosis, autophagy and senescence have also been reported to be responsible for the anti-tumor effects of MLN4924,18, 20, 31 as well as the inhibition of tumor angiogenesis and metastasis.22, 30 Our findings were not only consistent with the findings of other studies, but also reveal that MLN4924 induces G2/M arrest and apoptosis via the regulation of c-Jun degradation. This evidence concerns the gene JUN and neoplasm.