With prenatal HFD exposure known to increase anxiety-like behaviors in offspring (Bilbo and Tsang, 2010; Sullivan et al., 2010), and ENK in the PVN specifically shown to be a positive factor in inducing anxiety (Bilkei-Gorzo et al., 2008; Csabafi et al., 2011; Melo et al., 2014), this study tested whether prenatal CXCL12 administration has similar behavioral effects as the HFD. The gene discussed is CXCL12; the disease is Anxiety.