Compared with the pair-fed mice in the DEN + C group, the mice in the DEN + E group exhibited significantly higher ALT (Fig. 4a) and TG levels (Fig. 4b) and displayed more fat vacuoles in the liver (Fig. 4c), indicating more severe liver damage and pan-lobular steatosis in the DEN + E group. The gene discussed is GPT; the disease is steatosis.