Flow cytometry analysis showed that the proportions of early apoptotic cells (FITC Annexin V positive and PI negative) and late apoptotic cells (FITC Annexin V positive and PI positive) increased when NSCLC cells were cotreated with AZD4547 and beclin-1 siRNA (Fig. 6g-i). The gene discussed is ANXA5; the disease is non-small cell lung carcinoma.