In this study, splenocytes were cocultured together with irradiated 4TOO tumor cells and we observed that the percentage of specific IFN-γ producing CD4+ and CD8+ T cells was significantly higher after Mix + GalCer vaccination, compared to untreated mice (CD4 IFN-γ+: 3.75 ± 0.59% vs. 0.66 ± 0.18% p = 0.02; CD8 IFN-γ+: 10.61 ± 0.84% vs. 0.47 ± 0.03% p = 0.002) and α-GalCer treated animals (CD4 IFN-γ+: 3.75 ± 0.59% vs. 0.4 ± 0.25% p = 0.02; CD8 IFN-γ+: 10.61 ± 0.84% vs. 5.41 ± 1.46% p = 0.04) (Fig. 5c). This evidence concerns the gene CD8A and neoplasm.