We first investigated IKBKE expression levels in normal brain tissue, a low-grade malignant glioma cell line (H4) and high-grade malignant glioma cell lines (U87-MG, LN229, U251, LN308, A172 and SnB19) using real-time RT-PCR (Figure 1A) and western blotting (Figure 1B). This evidence concerns the gene IKBKE and malignant glioma.