EGFR and esophageal squamous cell carcinoma: We then used a quick in vitro function screening approach to examine whether these 14 genes have effects on malignant phenotypes of SCC cell lines, KYSE30 (ESCC), DaFu (HNSCC) and NCI-H520 (LUSCC) and found that knockdown of EGFR, BRD9 or PPFIA1 expression by short interfering RNA (siRNA) resulted in significant inhibition of cell proliferation (Fig. 3c and Supplementary Figs 18–20).