The results indicated that overexpressed-CsGRN PLC and RBE cell significantly increased vimentin, N-cadherin and β-catenin, and decreased ZO-1 compared with the control group, indicating that CsGRN is involved in the EMT process in CCA and HCC cells (Fig. 8a, b). The gene discussed is CDH2; the disease is hepatocellular carcinoma.