Although the present study did not directly address the effects of WSD and CR on fiber composition and metabolic properties of SM, the RNAseq analysis suggests that WSD induced the sustained downregulation of insulin signaling (IRS1), glycolytic (PFKM, PFKFB1), and mitochondrial (UCP3) genes and that this effect persisted after obesity was reversed with CR (Fig. 5). The gene discussed is IRS1; the disease is obesity due to melanocortin 4 receptor deficiency.