In addition, overexpression of STC1 significantly increased the protein expression of phospho-P65 in the cell nucleus, however PI3K inhibitor LY294002, AKT-shRNA and IκBα-shRNA decreased the level of phospho-P65 (Ser536) and the expression of phospho-P65 in cytoplasm in STC1 overexpressed HeLa (Figure 5D) and CaSki cells (Figure 5H) via ICC. The gene discussed is RELA; the disease is intrahepatic cholangiocarcinoma.