To further illuminate the relationship among STC1, PI3K/AKT, P65 (Ser536) and cell growth of Hela and Caski, we treated cells with PI3K inhibitor LY294002, AKT-shRNA and IκBα-shRNA in STC1 overexpressed cells, and detected the expression and phosphorylation levels of the signaling-related proteins in cervical cancer HeLa and CaSki cell lines. The gene discussed is RELA; the disease is cervical carcinoma.