To examine endogenous protein binding upon virus infection, FAF1 was immunoprecipitated from PR8-GFP or H1N1-infected HEK293T or RAW264.7 cells using an anti-FAF1 antibody, followed by immunoblotting with an anti-NLRX1 antibody, and band corresponds to NLRX1 was detected (Fig 6, panel F). This evidence concerns the gene NLRX1 and viral infectious disease.