To further validate our findings that mycobacterial lipids under WhiB3 control modulated the host cell cycle, we investigated if the infection of RAW264.7 macrophages with Mtb mutants for polyketides under WhiB3 control demonstrated similar alterations of the host cell cycle as MtbΔwhiB3. Macrophages were infected with Mtb, Mtb Tn:pks2 (necessary for SL-1 production), Mtb Tn:mas and Mtb Tn:ppsA (both required for PDIM production) for 12, 24, 36 and 48 h. The gene discussed is ARAF; the disease is infection.