According to the previous reports in which the proliferative effect of TNFα was examined, the effective concentration was 25 ng/ml in synovial fibroblasts [13], 10 ng/ml in bone marrow MSCs [23], 4–10 ng/ml in neural stem cells [24], 10 ng/ml in cardiac fibroblasts [25], and 25 ng/ml in leiomyoma cells [26], The effective concentration of TNFα for those cells was similar or close to ours. This evidence concerns the gene TNF and leiomyoma.