TF and anemia (phenotype): We observed that, compared to the CBA/Pk parent, the Char10C mice display a reduction in anemia phenotypes associated with the PklrG338D mutation including decreased splenomegaly, diminished circulating reticulocytes, increased density of mature erythrocytes, increased hematocrit, as well as decreased iron overload in kidney and liver and decreased serum iron and transferrin-bound iron (Figs 2, 3 and 4).