Mapping studies in an informative F2 cross generated between AcB62 (PklrI90N) and CBA/Pk (PklrG338D) identified the major genetic modifier of Pklr-associated malaria resistance to be linked to chromosome 9 (Char10; LOD = 7.24) with additional effects linked to segregation of functionally distinct mutant Pklr alleles mapping to chromosome 3 (LOD = 3.7)[19,27]. This evidence concerns the gene PKLR and malaria.