AKT1 and central nervous system cancer: We did not detect any change in the phosphorylation status of all the kinases examined, regardless of whether the expression of TRIM45 was upregulated or downregulated (Supplementary Figures 7A and B), suggesting that TRIM45 does not affect the PI3K/AKT, MAPK, and NF-κB signaling pathways in glioma cells.