As PI3K-Akt and Ras-MAPK signaling pathway are the most frequently activated signaling pathways involved in gliomagenesis and TRIM45 was also reported to be associated with PKC-mediated modulation of the ERK-JNK and NF-κB signaling pathway,19, 20 we examined the phosphorylation status of AKT, ERK, JNK, p38, and IKKβ in TRIM45-overexpressing or TRIM45 KO glioma cells. The gene discussed is TRIM45; the disease is central nervous system cancer.