Our results show that CD45+ haematopoietic induction from 400–2,000 colonies was significantly better than 150–500 (1.4 × ; Fig. 3g) and 400–500 (3.2 × ; Fig. 3g) colonies inferring that endogenous inhibitors were further mitigated by culturing HE cells as lower-coverage micropatterns. Here, PTPRC is linked to hereditary elliptocytosis.