The purpose of the present study was to assess the effects of pre-treated cannabidiol on cerebral infarction and TNFR1/NF-κB expression during ischemic injury in the rat model of transient focal cerebral ischemia to determine whether size of infarction is correlated to NF-κB and TNFR1 expression in cannabidiol received groups. The gene discussed is TNFRSF1A; the disease is brain infarction.