Cholestasis-associated hepatic steatosis18 was investigated, and while analysis of liver morphology by microscopy revealed little differences between control- and CA-fed mice, of both groups (Supplementary Fig. 3), we observed elevated hepatic triglycerides (TG), cholesterol and low-density/very low-density lipoprotein levels in WT mice following CA feeding (Fig. 3a–c), which were abborogated in TRPC5 KO mice; moreover, a significant difference was determined when compared to WT CA (P < 0.01; Fig. 3a). This evidence concerns the gene TRPC5 and cholestasis.