This prostate cancer cell line typically expresses a number of murine prostate-associated antigens, such as PAP, PSCA and STEAP1, and has been used for testing protective efficacy of cancer vaccines targeting those antigens [23, 24]; however, TRAMP-C1 cells have not been characterized in terms of 5T4 expression. This evidence concerns the gene TPBG and Familial prostate cancer.