To verify whether autophagosome formation was increased in cachectic muscle of LLC tumor-bearing mice in a TLR4-dependent manner, we overexpressed GFP-LC3 in mouse TA and observed increased GFP-LC3-labeled autophagosomes in LLC tumor-bearing WT but not TLR4−/− mice (Fig. 4B). The gene discussed is MAP1LC3A; the disease is neoplasm.