TRPV1 and ethylmalonic encephalopathy: The level of TRPV1 gene transcription, analyzed by Q-PCR and referenced to 18S rRNA gene, was not significantly different between groups: 1.93 (0.86–2.90) in EE patients, 1.40 (1.18–2.76) in NERD patients and 0.92 (0.42–2.54) in HVs (Figure 4).