In the present study, SFRP1, SFRP2, SFRP5, DKK2, DKK3, mir34b/c, RASSF1A, IGFBP7, CDKN2A, and MLH1 were examined for DNA methylation status in isolated cancerous crypts from CRC and the surrounding normal crypts. The gene discussed is CDKN2A; the disease is colorectal carcinoma.