In this study, significant up-regulation in the levels of aldose reductase (p < 0.01) and sorbitol dehydrogenase (p < 0.01) with concomitant depletion of glyoxalase-I (p < 0.01) were observed in the myocardial tissue homogenate of T2D rats (Figure 4). This evidence concerns the gene AKR1B1 and type 2 diabetes mellitus.