It is not entirely clear whether the protective effect of scopolin against HFD-induced hepatic steatosis is mediated primarily through the allosteric activation of SIRT1 in vivo. It is intriguing that besides the SIRT1 activity, the abundance of hepatic SIRT1 protein and mRNA (Fig. 5B and C) was also pronounced in mice treated with scopolin. Here, SIRT1 is linked to fatty liver disease.