To further investigate whether IL-1R8 up-regulation in tumor cells could modulate NK cell activation, NKL cells were cultured in the presence of either HB4aHER2+ or HB4aHER2+/IL1R8KD cells and IFN-γ levels in the culture supernatant were used as a surrogate marker of NK cell activation. This evidence concerns the gene SIGIRR and neoplasm.