The results of Sp1-regulated CYP17A1 transcription are not the same with that in human adrenal NCI-H295A cells in which the transcription of CYP17A1 gene is majorly contributed by NFIC, Sp1 and Sp3.24 They also identified Sp1/Sp3, and NFIC regulate CYP17A1 transcription through binding to the −227/−184 and −107/−85 regions, respectively.24 However, in the complementary DNA microarray database of glioma, GSE4290 in Figure 2a, Sp1 expression, neither NFIC nor Sp3, significantly correlates with CYP17A1 expression, suggesting that Sp1, not NFIC and Sp3, regulates CYP17A1 expression for glioma. Here, SP1 is linked to central nervous system cancer.