AKT1 and neoplasm: Arachidonic acid is used to activate TRPV4 channel as it is a well-known TRPV4 agonist.14 In fact, others have demonstrated tumor-derived endothelial cell migration mediated by TRPV4 via arachidonic acid-activated actin remodeling.14 The results demonstrated that AKT is required for TRPV4-mediated wound healing and transendothelial migration (Supplementary Figure 3).