In addition, AKT has also been shown to contribute to tumor invasion and metastasis by promoting the secretion of tumor-associated proteins.24, 25 Since secreted proteins are both critical mediators of metastasis and attractive drug targets, we employed ITRAQ-based mass spectrometry to profile the levels of secreted proteins that are specifically upregulated by TRPV4 upon 4α-PDD treatment. Here, TRPV4 is linked to neoplasm.