To test whether vagotomy or double deletion of Chrna7 and Itgam impaired serine473 phosphorylation of AKT1 in recruited neutrophils and monocytes during E. coli infection, we IT challenged sham, vagotomized and Chrna7−/−Itgam−/− mice with E. coli. The gene discussed is CHRNA7; the disease is escherichia coli infection.