In this study we wanted to investigate in detail the role of Notch signaling in neuroblastoma and we analyzed the role of Notch pathway components, three receptors (Notch1, Notch 2 and Notch 3) and five canonical ligands (Delta-like 1, Delta-like 2, Delta-like 4, Jagged 1 and Jagged 2), in three neuroblastoma cell lines. Here, NOTCH1 is linked to neuroblastoma.