BRD4 and nut midline carcinoma: Furthermore, we also observed upregulation of autophagy and lysosomal gene expression upon BRD4-NUT inhibition by NUT siRNA and JQ1 and an increase in lysosomal protein levels and LysoTracker+ acidic compartments in BRD4-NUT knockdown NMC cells (Figures 3F and 3G; Figures S3B and S3C), suggesting that BRD4-NUT also suppresses the autophagy-lysosome pathway in NMC.