To assess whether AMPK activation also affects the decrease in p-mTOR, p-p70s6K, and survivin elicited by sestrin 2 overexpression, western blot analysis was conducted in SW620 CRC cells treated with compound C. Data showed that the levels of these three proteins were increased in the LV-sestrin 2 group after AMPK inhibition, while no changes were elicited by compound C treatment in control LV-NC cells (Figure 5). The gene discussed is RPS6KB1; the disease is colorectal carcinoma.