Three experimental groups were established using cultured SW620 and LoVo CRC cells: 1) LV-sestrin 2 (cells with stable sestrin 2 overexpression); 2) LV-NC (negative control cells transduced with a non-targeted lentiviral vector); and 3) BC (untransfected, blank control cells). Here, SESN2 is linked to colorectal carcinoma.