Here, we lentiviral-transduced the same CD19 CAR into Dynabead T cells, OKT3-28BB-86BBL RNA-T cells, and OKT3/IL-2 T cells and tested their anti-leukemia activity in the Nalm6 model, and the OKT3/IL-2 T cells were still inferior to the CD3/CD28 Dynabead T cells in controlling leukemia. This evidence concerns the gene CD28 and leukemia.