The early syphilis diagnostics were based on a single recombinant antigen, where sensitivities and specificities of Tp15, Tp17, and Tp47 were 100% and 96%; 100% and 100%; 100% and 20%, respectively [28], while assays with two or three antigen combinations resulted in the improvement of diagnostic assay [29]. Here, CDKN2B is linked to syphilis.