In the current study, we found the expression of CD133 was negatively correlated with WWOX and E-cadherin, and a positive correlation between WWOX and E-cadherin in colorectal samples, this indicates a definite relationship between the three biomarkers in colorectal cancer, facilitating physicians to predict the progression and prognosis of colorectal cancer. The gene discussed is CDH1; the disease is colorectal cancer.