In addition to TRPV4’s effect on the cytokine/inflammatory changes in ARDS, TRPV4 actions can induce lung endothelial barrier dysfunction in vitro and in vivo, as well as cause disruption of alveolar type I epithelial cells leading to lung vascular leak and alveolar edema (9, 29). This evidence concerns the gene TRPV4 and acute respiratory distress syndrome.