Real-time PCR and western blotting analysis of CKAP2 levels revealed that CKAP2 expression was decreased by 94.8 ± 1.2% and 77.7 ± 1.5% in HeLa cells and 97.5 ± 3.9% and 75.4 ± 1.2% in C-33A cells respectively following infection with pLKO.1-EGFP-CKAP2 shRNA-1 compared with control (Fig. 2C and D). The gene discussed is CKAP2; the disease is infection.