Our results demonstrate that the use of a Synthemax xeno-free surface enables thepropagation of glioblastoma-derived cells as a monolayer culture without serumsupplementation, and the cells were positive for the examined AAAs(IL13Rα2, Fra-1), the analysed CSCs markers and the selected markers ofEMT (Figures 1 and 2). The gene discussed is IL13RA2; the disease is glioblastoma.